The Synapt QTOF is a next generation electrospray TOF instrument and is our most recent acquisition. In addition to high mass accuracy and resolution, the Synapt is equipped with an ion mobility chamber. Ion mobility measurements facilitate determenation of molecular cross-sectional area. This can be used to detect the difference between structural isomers of a single chemical formula, measure the folded or unfolded state of proteins, and detect assembly states of macromolecular complexes. Sample introduction can be performed via classic electrospray, online nanospray with direct infusion or HPLC, and offline nanospray using as little as microliters of sample. The high performance, sensitivity, and ion mobility capability of the Synapt makes it unrivaled for the biophysical study of complex systems.